膜片钳技术是通过记录离子通道的离子电流来反映细胞膜上离子通道的特性,可以对通道的电导、动力学特性、离子选择性、药理学特性以及通道的调节机制等方面进行深入细致的研究,从而为研究细胞膜的生理学特性,鉴别细胞膜表面蛋白及其功能提供了线索。本文就膜片钳技术的原理、记录方法等进行综述,阐述了膜片钳技术在口腔肿瘤细胞研究中的应用价值及前景。 Patch clamp technology is the ion channel current ion channel to reflect the characteristics of ion channels on the cell membrane, the channel conductivity, kinetic properties, ion selectivity, pharmacological properties, as well as the channel adjustment mechanism and other aspects of intensive study , Thus providing clues for studying the physiological characteristics of cell membranes and identifying cell membrane surface proteins and their functions. In this paper, the principle of patch clamp technique, recording methods and so on are reviewed, and the application value and prospect of patch clamp technique in the study of oral tumor cells are expounded.