HBV表面蛋白的preS区具有强的免疫原性,对PreS_1特异的T辅助细胞可辅助主蛋白特异的B细胞活化增殖,克服对主蛋白的无反应性,目前带有preS区的基因工程重组疫苗正在研究当中。preS_1和preS_2区各自带有T细胞和B细胞识别位点,PreS_1(21～47)为HBV结合肝细胞受体的位点,抗preS_1(21～47)肽的抗体具有保护作用。根据preS抗原的上述特点,我们选择了preS基因,构建了pCMV_4preS和 The preS region of HBV surface protein has strong immunogenicity. PreS1-specific T helper cells can assist the activation of primary protein-specific B cell proliferation, to overcome the non-response to the main protein, the current genetic engineering with preS region recombinant vaccine Is studying. PreS_1 and preS_2 regions contain T cell and B cell recognition sites respectively. PreS_1 (21-47) is a site of HBV-binding hepatocyte receptors, and antibodies against preS_1 (21-47) peptides have protective effects. Based on the above characteristics of preS antigen, we chose the preS gene to construct pCMV_4preS and