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目的：研制与膀胱移行上皮癌（ＴＣＣ）特异结合的单克隆抗体Ｌ４Ｂ４。方法：用免疫组化法评价Ｌ４Ｂ４，并对荷人ＴＣＣ裸鼠行放射免疫显像（ＲＩＩ）。结果：免疫组化研究显示：Ｌ４Ｂ４对ＴＣＣ具有很高的特异性（２３／２４），而很少与其他恶性肿瘤（２／２４）及良性肿瘤（０／７）相结合。荷瘤裸鼠ＲＩＩ研究显示：注入１２５Ｉ－Ｌ４Ｂ４后第３天及第５天可清晰显示肿瘤影像，第５天的Ｔ／ＮＴ＞４。结论：Ｌ４Ｂ４可在膀胱癌的ＲＩＩ研究中发挥作用，值得进一步研究。
Objective: To develop a monoclonal antibody L4B4 that specifically binds to bladder transitional cell carcinoma (TCC). Methods: L4B4 was evaluated by immunohistochemistry and radioimmunoimaging (RII) was performed on nude mice bearing TCC. Results: Immunohistochemistry showed that L4B4 had a high specificity for TCC (23/24), but seldom associated with other malignancies (2/24) and benign tumors (0/7). The tumor-bearing nude mice RII study showed that tumor images were clearly visible on day 3 and day 5 after 125I-L4B4 injection, with T / NT> 4 on day 5. Conclusion: L4B4 can play a role in RII of bladder cancer and deserves further study.