论文部分内容阅读
大豆异黄酮是一种广泛应用的保健性活性物质,近年来已成为衡量大豆品质的重要指标之一。异黄酮合酶是大豆异黄酮合成途径中的关键酶基因之一,其在植物中的表达效率直接影响异黄酮含量。为进一步验证该基因的功能并获得高异黄酮稳定遗传转基因植株,本试验基于已有的IFS转基因材料开展研究。将其扩繁至T2代,考种分析植株农艺性状,发现转基因植株的性状指标未发生明显变化,PCR鉴定IFS转基因后代的结果显示:在125株转基因植株中,60株为阳性,占比48%,说明IFS在后代中可稳定遗传。选取IFS转基因吉林35、Willimas 82品种T2代的41株,利用改良后的三波长法测定籽粒中大豆异黄酮的含量,结果显示:IFS转基因植株的平均异黄酮含量为1.2 mg·g~(-1);其中15株的异黄酮含量高于非转基因植株,占比达到36.6%,说明从转入IFS基因转基因大豆能够筛选出高异黄酮植株。本研究获得了稳定遗传的高异黄酮植株,为大豆遗传育种提供优异的种质资源;改良后的三波长法较原有方法更为精准、快速。
Soy isoflavone is a widely used health care active substance, which has become one of the most important indexes to measure the quality of soybean in recent years. Isoflavone synthase is one of the key enzyme genes in the soybean isoflavone synthesis pathway. The efficiency of its expression in plants directly affects the isoflavone content. To further validate the function of this gene and obtain transgenic plants with high isoflavone stability, the present study was based on existing IFS transgenic material. The results of PCR identification of progeny of IFS showed that among the 125 transgenic plants, 60 were positive, accounting for 48 %, Indicating that IFS can be stably inherited in offspring. The results showed that the average isoflavone content of IFS transgenic plants was 1.2 mg · g ~ (-1), and the average isoflavone content in the seeds of IFS transgenic plants was 1.2 mg · g ~ (-1) 1). Among them, 15 had higher isoflavone content than non-transgenic plants, accounting for 36.6% of the total, indicating that high isoflavone plants can be screened from transgenic soybean transformed to IFS gene. In this study, we obtained stable isoflavone-rich plants and provided excellent germplasm resources for soybean genetic breeding. The improved three-wavelength method is more accurate and rapid than the original method.