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目的:研究动脉粥样硬化过程中低密度脂蛋白免疫复合物(LDL-IC)对单核细胞来源的巨噬细胞内脂质含量的影响。方法:以佛波酯刺激分化的THP-1细胞作为单核细胞来源巨噬细胞模型。固定后的细胞以苏丹Ⅳ染色,观察细胞内脂质含量变化。通过胆固醇酶联法测定细胞内胆固醇含量。结果:与LDL-IC孵育能使单核细胞来源巨噬细胞内胆固醇堆积,呈现泡沫细胞的外观。细胞内胆固醇水平随着LDL-IC的浓度增加与作用时间的延长而升高。在4组平行对照实验中,LDL-IC组细胞内胆固醇水平明显高于阴性对照组、LDL组和IgG-IC组(P<0.01)。结论:LDL-IC能通过增加细胞内脂质而在动脉粥样硬化过程中发挥作用。
Objective: To study the effect of low density lipoprotein (LDL-IC) on lipids in monocyte-derived macrophages during atherosclerosis. Methods: Differentiated THP-1 cells stimulated by phorbol ester as a monocyte-derived macrophage model. Immobilized cells were stained with Sudan IV to observe the changes of intracellular lipid content. Cholesterol enzymatic determination of intracellular cholesterol content. Results: Incubation with LDL-IC caused the accumulation of cholesterol in monocyte-derived macrophages, which showed the appearance of foam cells. Intracellular cholesterol levels increased with increasing concentrations of LDL-IC and prolongation of action time. In four parallel control experiments, the level of intracellular cholesterol in LDL-IC group was significantly higher than that in negative control group, LDL group and IgG-IC group (P <0.01). Conclusion: LDL-IC can play an important role in atherosclerosis by increasing intracellular lipids.