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在紫色土上,探究接种土著AMF(indigenous arbuscular mycorrhizal fungi)及不同形态氮肥施用对间作大豆Glycine max生长及氮利用的影响,为提高间作大豆对土壤不同形态氮素的吸收与利用,减少土壤无机氮残留提供理论依据。采用盆栽试验,设2种种植方式(大豆单作和玉米/大豆间作),不同丛枝菌根真菌处理[不接种(NM)、接种土著AMF]和3个氮处理[不施氮(N0)、施无机氮(ION120)、施有机氮(ON120)],以期揭示土著AMF和不同形态氮施用对间作大豆生长及氮素吸收利用的影响。结果表明:与N0相比,施ION120和ON120处理显著增加了土壤无机氮的累积量。NM条件下,无论何种施氮处理的间作土壤NH_4~+-N、NO_3~--N含量均低于单作,其中当接种土著AMF时,与单作相比,间作对减少土壤无机氮的积累能力得到进一步加强。无论单作或是间作,相同菌根处理下,ION120和ON120处理的大豆地上部和根系生物量,大豆地上部和根系氮含量及大豆地上部和根系氮吸收量均不同程度地高于N0处理,其中间作-土著AMF条件下,ION120处理的根系生物量、根系氮含量及氮吸收量均显著高于ON120处理。间作-ION120条件下,土著AMF处理的大豆地上部氮含量、吸收量及根系氮含量、氮吸收量较NM处理分别提高了9.8%、69.8%和8.1%、54.8%,四者差异均达到显著水平。除根系氮吸收量外,地上部氮含量、氮吸收量及根系氮含量均在间作-土著AMF-ION120处理下显著提高,间作与土著AMF互作优势明显。间作-土著AMF条件下,ION120和ON120处理的大豆根系氮吸收效率高于N0处理,分别提高了2%和6%。总体来看,土著AMF与ION120氮肥施用对促进间作大豆生长与提高氮素利用率尤为明显,可望减少土壤氮素残留而减轻氮素流失的风险。
In purple soil, the effects of inoculation AMF (indigenous arbuscular mycorrhizal fungi) and different forms of nitrogen fertilizer on the growth and nitrogen utilization of intercropped soybean Glycine max were studied. In order to increase the absorption and utilization of intercropped soybean on different forms of nitrogen in soil, Nitrogen residues provide the theoretical basis. A pot experiment was conducted to investigate the effect of AMF treatment on AMF and three nitrogen treatments [N0 (N0)] in two different planting modes (monoculture of soybean and intercropping of corn and soybean), arbuscular mycorrhizal fungi (AM) , Application of organic nitrogen (ON120) and organic nitrogen (ON120)] in order to reveal the effects of indigenous AMF and different forms of nitrogen application on soybean growth and nitrogen uptake and utilization. The results showed that compared with N0, application of ION120 and ON120 significantly increased soil inorganic N accumulation. NM, the contents of NH_4 ~ + -N and NO_3 ~ -N in intercropped soils under any nitrogen application were lower than that of single intercropping. When AMF was inoculated, intercropping could reduce soil inorganic nitrogen The accumulation capacity has been further strengthened. Whether single or intercropped, the aboveground and root biomass of soybean with ION120 and ON120 treatment, aboveground and root nitrogen content of soybean and aboveground and root nitrogen absorption of soybean were higher than N0 treatment under the same mycorrhizal treatment , The root biomass, root nitrogen content and nitrogen uptake of ION120 were significantly higher than those of ON120 under intercropping-indigenous AMF. Under intercropping -ION120, the contents of nitrogen, uptake and root nitrogen, and nitrogen uptake of above-ground AMF increased by 9.8%, 69.8% and 8.1% and 54.8%, respectively, compared with those of NM treatment Level. In addition to the root nitrogen uptake, aboveground nitrogen content, nitrogen uptake and root nitrogen content were significantly increased in the intercropping AMF-ION120 intercropping process, intercropping with indigenous AMF obvious advantages. Under AMF conditions, the nitrogen uptake efficiency of soybean roots treated with ION120 and ON120 was higher than that of N0 treatment by 2% and 6% respectively. Overall, the application of indigenous AMF and ION120 nitrogen fertilizer is particularly significant for promoting the growth of intercropped soybean and increasing the nitrogen utilization efficiency, and it is expected to reduce the residual nitrogen in soil to reduce the risk of nitrogen loss.