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目的:利用Box-Behnken响应面法对超声波辅助提取银耳多糖工艺进行优化。方法:在单因素试验基础上,选择超声波功率、超声时间和液料比为考察因素,以银耳多糖提取率为评价指标,采用Box-Behnken响应面法考察各个因素及其交互作用对银耳多糖提取率的影响。结果:最佳提取工艺为:超声波功率为360 W,超声时间为23 min,液料比为45∶1 ml·g~(-1)。在优化提取工艺参数条件下提取6批银耳多糖,平均提取率为20.4%±1.8%(n=6)。结论:利用Box-Behnken响应面法优化超声波辅助提取银耳多糖工艺,方法简便,预测性良好。
OBJECTIVE: To optimize the technology of ultrasonic extraction of Tremella fuciformis with Box-Behnken response surface method. Methods: Based on the single-factor experiments, ultrasonic power, ultrasonic time and liquid to material ratio were selected as the investigation factors. The extraction rate of Tremella fuciformis was used as evaluation index, and the factors of Box-Behnken response surface method Rate of impact. Results: The optimum extraction conditions were: ultrasonic power of 360 W, ultrasonic time of 23 min and liquid to liquid ratio of 45:1 ml · g -1. Six batches of Tremella fuciformis polysaccharides were extracted under optimized extraction conditions with an average extraction rate of 20.4% ± 1.8% (n = 6). Conclusion: The Box-Behnken response surface method was used to optimize the ultrasonic-assisted extraction of Tremella fuciformis. The method is simple and predictive.