中性粒细胞(NC)对囊性纤维化(CF)的肺脏具强有力的破坏作用。嗜酸性细胞(EC)也有破坏的可能,故而作者对CF者EC的作用进行了调查研究。 方法测定42例CF者的血清和其中10例痰中EC、嗜酸细胞正性蛋白(ECP)含量,后者作为嗜酸性细胞活性标志物。也对NC的单胺氧化酶(MPO)活性程度进行测评。ECP和MPO是以双倍抗体的放射免疫法测定。设非CF的30名健康对照者进行检测对照。还采用Shwachman-Kulczycki评分法评估CF的临床严重程度,并判定其肺功能情况。 结果 患者的特征 Shwachman-Kulczycki评分 Neutrophils (NC) have a strong destructive effect on lungs of cystic fibrosis (CF). Eosinophils (EC) also have the possibility of destruction, so the authors investigated the role of CF EC. Methods The serum levels of EC and eosinophilic protein (ECP) in sputa of 42 patients with CF were determined. The latter was used as eosinophil activity marker. The degree of monoamine oxidase (MPO) activity of NC was also evaluated. ECP and MPO are double antibody radioimmunoassay. A non-CF 30 healthy controls were tested for control. The Shwachman-Kulczycki score was also used to assess the clinical severity of CF and to determine its lung function. Results Patient characteristics Shwachman-Kulczycki score