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采用不同浓度镧来固体培养美洲商陆毛状根,并采用分光光度法来测定毛状根的商陆皂苷含量、抗氧化酶SOD和POD活性及苯丙氨酸裂解酶(PAL)酶活性,以探讨镧对美洲商陆毛状根生长及其次生代谢影响的生理机制。结果表明,镧浓度低于10.0 mg·L~(-1)可促进毛状根的生长,其中以5.0 mg·L~(-1)镧处理的毛状根生物量最大,而高于20.0 mg·L~(-1)镧则抑制毛状根生长,但其皂苷含量最高;与对照相比,低于10.0 mg·L~(-1)镧可提高毛状根SOD活性,其中以1.0 mg·L~(-1)镧的SOD活性最高;而镧浓度为0.5 mg·L~(-1)时,毛状根的POD活性最高,大于0.5 mg·L~(-1)时,POD活性随着镧浓度的升高而降低;而毛状根PAL活性及其皂苷含量则随着镧浓度增大而逐渐提高。表明镧可能通过提高毛状根的抗氧化酶和PAL活性而影响其生长和皂苷的合成。
Different concentrations of lanthanum were used to solidly cultivate hairy roots of Pseudostellaria polytricha, and the contents of saponins, SOD and POD activities and PAL activity of hairy roots were determined by spectrophotometry Physiological Mechanism of Lanthanum on Hairy Roots and Its Secondary Metabolism of. The results showed that the growth of hairy roots was promoted when the concentration of lanthanum was less than 10.0 mg · L -1, and the biomass of hairy roots treated with 5.0 mg · L -1 lanthanum was the highest, while the concentration of lanthanum was above 20.0 mg L ~ (-1) lanthanum inhibited hairy root growth but had the highest saponin content. Compared with the control, Lanthanum lanthanum (10.0 mg · L -1) increased SOD activity in hairy roots, with 1.0 mg · L ~ (-1) lanthanum had the highest activity of SOD. When lanthanum concentration was 0.5 mg · L -1, POD activity of hairy roots was the highest. Above 0.5 mg · L -1, POD activity Decreased with the increase of lanthanum concentration, while PAL activity and saponin content of hairy roots gradually increased with the increase of lanthanum concentration. It is suggested that lanthanum may affect its growth and the synthesis of saponins by increasing the antioxidant enzymes and PAL activities of hairy roots.