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作者分别采用脂多糖(LPS)和凝集素刺激T细胞与单核细胞,在活化的细胞表面检测到preS_1受体,表明非特异性刺激物LPS和ConA均能诱导这两类细胞表达preS_1受体。在研究活化细胞表面preS_1受体的过程中,作者利用亲和层析结合ELISA夹心法首次发现自细胞介素-6(IL-6)与preS_1可发生特异性结合,并进一步用微孔超滤加ELISA证实了preS_1上的IL-6结合位点。许多细胞能产生IL-6,表明乙型肝炎病毒(HBV)对细胞的泛嗜性。虽然有的细胞(如PBMC、前
The authors used lipopolysaccharide (LPS) and lectin to stimulate T cells and monocytes, respectively, and detected the preS_1 receptor on the surface of activated cells, indicating that both nonspecific stimulators LPS and ConA can induce these two kinds of cells to express preS_1 receptor. In the process of studying the preS_1 receptor on the surface of activated cells, the authors first discovered that IL-6 and preS_1 can be specifically bound by affinity chromatography and ELISA sandwich method, and further microporous ultrafiltration Addition of ELISA confirmed the IL-6 binding site on preS_1. Many cells produce IL-6, indicating the panotropic nature of Hepatitis B virus (HBV) on cells. Although some cells (such as PBMC, before