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目的:探讨何首乌有效成分二苯乙烯甙对神经细胞保护作用的机制。方法:使用不同浓度的二苯乙烯甙与SK-N-SH细胞预孵育6h与24h,对比分析不同浓度的二苯乙烯甙的LDH漏出率以及细胞存活率。结果:孵育6h后,Aβ25-35引起的细胞存活率下降情况受到拮抗,随着二苯乙烯甙浓度的增加,LDH漏出率不断下降;孵育24h后,200μmol/L的二苯乙烯甙的保护作用最佳。结论:二苯乙烯甙能有效拮抗因Aβ25-35引起的细胞存活率下降、乳酸脱氢酶漏出增多,对防治老年性痴呆引起的神经功能下降具有良好的前景。
Objective: To investigate the protective effect of stilbene glucoside on nerve cells. Methods: Different concentrations of stilbene and SK-N-SH cells were pre-incubated for 6h and 24h, respectively. The LDH leakage rate and cell viability of different concentrations of stilbene glycosides were compared. Results: After 6 hours of incubation, the decline of cell viability induced by Aβ25-35 was antagonized. With the increase of the concentration of stilbene glucoside, the leakage rate of LDH decreased continuously. After 24h incubation, the protective effect of stilbene glycoside was 200μmol / L optimal. CONCLUSIONS: Stilbene glycoside can effectively prevent the decline of cell viability induced by Aβ25-35 and increase the leakage of lactate dehydrogenase, which has a good prospect of preventing neurological decline caused by senile dementia.