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虫荧光素[D-(-)2-(6’-羟基-2’-苯并噻唑基)-△~2-噻唑啉-4-羧酸]简称LH_2。 Bitler和McElroy于1957年从美洲荧火虫中分离提纯,1961年White等合成了LH_2,并报告了其理化性质。虫荧光素是虫荧光素酶的重要底物,是生物发光法测定ATP必不可少的试剂。由于ATP是机体内重要的代谢调节物质和能量“通货”,与生命活动密切相关。因此近10余年来在基础与临床医学中广泛开展ATP及通过偶联产生与消耗ATP的物质(如ADP、AMP、CP等)的检测。虫荧光素酶发光系统对
Fluorescein [D - (-) 2- (6’-hydroxy-2’-benzothiazolyl) - △ ~ 2-thiazoline-4-carboxylic acid] referred to as LH_2. Bitler and McElroy were isolated and purified from the American firefly in 1957. In 1961, White et al. Synthesized LH_2 and reported its physico-chemical properties. Fluorescein is an important substrate for luciferase and is an indispensable reagent for the determination of ATP by the bioluminescence method. As ATP is an important metabolic regulator of the body and energy “currency”, and life activities are closely related. Therefore, more than 10 years in the basic and clinical medicine in the extensive ATP and through the coupling and consumption of ATP substances (such as ADP, AMP, CP, etc.) detection. Luciferase luciferase system pair