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目的:建立一种莽吉柿果皮中3个氧杂蒽酮类化合物的RP-HPLC含量测定方法。方法:色谱柱为Waters XTerra RP18色谱柱(150 mm×4.6 mm,5μm),以水-0.5%醋酸(A)、甲醇-0.5%乙酸(B)为流动相,梯度洗脱,流速为1.0 mL·min-1,检测波长为317 nm,柱温为30℃。结果:3-异倒捻子素、α-倒捻子素、β-倒捻子素浓度分别在21.3～213,107.7～1077,10.6～106μg·mL-1范围内线性关系良好(r≥0.9991);平均回收率分别为102.6%,98.5%,103.5%,RSD分别为1.7%,1.2%,2.0%(n=6)。结论:所建立的方法便捷、准确,重复性好,可以为莽吉柿果皮的质量控制提供一定的参考。
OBJECTIVE: To establish a RP-HPLC method for the determination of three xanthone compounds in the mango peel. METHODS: The column was a Waters XTerra RP18 column (150 mm × 4.6 mm, 5 μm) with a gradient of 1.0 mL of water and 0.5 mL of acetic acid (A) and methanol-0.5% · Min-1, the detection wavelength was 317 nm and the column temperature was 30 ℃. Results: The concentrations of 3-isogin, α-mangostin and β-mangostin were good linearity (r≥0.9991) in the range of 21.3 ~ 213,107.7 ~ 1077,10.6 ~ 106μg · mL- They were 102.6%, 98.5% and 103.5% respectively, with RSDs of 1.7%, 1.2% and 2.0%, respectively (n = 6). Conclusion: The established method is convenient, accurate and reproducible, which can provide some references for the quality control of Mangosteen peel.