高分子和正负离子表面活性剂混合物可形成一种新型双水相体系.研究蛋白质在溴化十二烷基三乙铵/十二烷基硫酸钠与聚氧乙烯(EO)-聚氧丙烯(PO)嵌段共聚物(EO_(20)PO_(80))共组双水相体系中的分配.通过在高分子接上亲和配基,研究蛋白质在带有亲和配基高分子的双水相体系中的分配.将表面活性剂富集相稀释或加热高分子富集相,又可形成新的双水相体系,由此可进行蛋白质的多步分配.在蛋白质的分配完成之后,通过将表面活性剂富集相进一步稀释或将高分子富集相加热至高分子浊点以上可将表面活性剂和高分子与目标蛋白质分离.正负离子表面活性剂和高分子还可以循环使用. A new type of aqueous two-phase system was formed by the mixture of polymer and cationic surfactant.The effects of sodium dodecyltriethylammonium bromide / sodium lauryl sulfate and polyoxyethylene (PO) -polyoxypropylene (PO ) Block copolymer (EO_ (20) PO_ (80)) in aqueous two-phase system.According to the affinity of the polymer attached to the ligand, the study of protein with affinity ligand polymer in the double water Phase system The surfactant-rich phase is diluted or heated to enrich the polymer-rich phase and a new aqueous two-phase system is formed whereby multi-step protein partitioning can be carried out.When protein distribution is complete, The surfactant-enriched phase is further diluted or the polymer rich phase is heated to above the cloud point of the polymer to separate the surfactant and the polymer from the target protein.The cationic and anionic surfactants and polymers can also be recycled.