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目的:建立测定人参强心滴丸中华蟾酥毒基和脂蟾毒配基含量的HPLC方法。方法:采用Diamonsil C18(200 mm×4.6 mm,5μm)色谱柱,流动相为0.5%磷酸二氢钾溶液-乙腈(45∶55,用磷酸调节pH为3.2),流速1.0 mL·min-1,检测波长为296 nm,柱温为室温。结果:华蟾酥毒基、脂蟾毒配基线性范围分别为2.54～50.8μg·mL-1(r=0.9999)和2.48～49.6μg·mL-1(r=0.9999),平均回收率(n=6)分别为99.9%(RSD=1.0%)和99.5%(RSD=0.59%)。结论:本法简便准确,专属性强,可作为人参强心滴丸中华蟾酥毒基和脂蟾毒配基含量的控制方法。
OBJECTIVE: To establish an HPLC method for the determination of cinobufagin and resibufogenin in ginseng Qixin pills. METHODS: Diamonsil C18 (200 mm × 4.6 mm, 5 μm) column was used. The mobile phase consisted of 0.5% potassium dihydrogen phosphate solution - acetonitrile (45:55, adjusted to pH 3.2 with phosphoric acid) at a flow rate of 1.0 mL · min- The detection wavelength was 296 nm and the column temperature was room temperature. Results: The linear ranges of cinobufagin and resibufogenin were 2.54-50.8μg · mL-1 (r = 0.9999) and 2.48-49.6μg · mL-1 (r = 0.9999) respectively. The average recoveries were 6) were 99.9% (RSD = 1.0%) and 99.5% (RSD = 0.59%), respectively. Conclusion: This method is simple, accurate and specific. It can be used as a control method for the contents of Chinese toad venom and resibufogenin of ginseng Qixin pills.