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目的为了解亚临床感染弓形虫的孕妇，母婴之间垂直传播情况。方法采用聚合酶链技术（ＰＣＲ）分别检测母婴血弓形虫ＤＮＡ（Ｔｏｘｏ－ＤＮＡ）及羊水Ｔｏｘｏ－ＤＮＡ．结果１．对３０例孕晚期孕妇检测羊水Ｔｏｘｏ－ＤＮＡ与其新生儿血Ｔｏｘｏ－ＤＮＡ，其检测结果无显著差异，Ｐ＞０．０５。２．孕中晚期组孕妇感染弓形虫后，其胎儿感染率明显高于孕早期组，Ｐ＜０．０５。结论孕中晚期孕妇感染弓形虫后，易于传染给胎儿且感染弓形虫为孕妇若能维持到足月分娩，出生的胎儿多为无临床症状的先天性弓形虫病（实验实诊断为阳性），这组胎儿有可能于数年后发生远期并发症；由于羊水易于在孕中晚期获取，故检测羊水Ｔｏｘｏ－ＤＮＡ将有利于先天性弓形虫病的早期诊断及早期治疗，从而控制先天性弓形虫病儿的出生。
Objectives To understand the vertical transmission between pregnant women and mothers and babies of subclinical Toxoplasma gondii infection. Methods Polymerase chain reaction (PCR) was used to detect Toxo-DNA and amniotic fluid Toxo-DNA in maternal and neonates respectively. Results 1. 30 cases of pregnant women in the third trimester detection of amniotic fluid Toxo-DNA and neonatal blood Toxo-DNA, the test results showed no significant difference, P> 0.05.2. In the second trimester pregnant women infected with Toxoplasma gondii, the fetus infection rate was significantly higher than the early pregnancy group, P <0.05. Conclusion Pregnant women in the second trimester of pregnancy infected with Toxoplasma gondii, easy to infect the fetus and infected with Toxoplasma gondii if pregnant women can be maintained to full-term delivery, the birth of the fetus are mostly clinical symptoms of congenital toxoplasmosis (the actual diagnosis is positive) This group of fetuses may have long-term complications after a few years. Because amniotic fluid is easy to obtain in the second trimester of pregnancy, detection of amniotic fluid Toxo-DNA will be helpful for the early diagnosis and early treatment of congenital toxoplasmosis, thus controlling the congenital arch The birth of a worm sick child.