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目的：了解透明质酸刺激因子（ＨＡＳＦ）对三维培养的瘢痕成纤维细胞收缩特性的影响．方法：将提纯的兔源性ＨＡＳＦ加入以人皮肤胶原为支架的人增生性瘢痕成纤维细胞三维培养系统（ＦＰＣＬ），通过计算ＦＰＣＬ的收缩指数，用放免法测定其中ＨＡ的含量，就ＨＡＳＦ对三维培养的瘢痕成纤维细胞收缩性，快速收缩和ＨＡ合成的影响及其相关性进行初步研究．结果：三维培养系统中ＨＡＳＦ浓度≥１×１０－３ｇ／Ｌ时，ＨＡＳＦ与细胞介导的ＦＰＣＬ最大收缩指数有明显的剂量—效应关系，ＦＰＣＬ的收缩指数与对照组之间的差异非常显著（Ｐ＜０．０１）；ＨＡＳＦ对ＦＰＣＬ的快速收缩无影响（Ｐ＜０．０５）；ＦＰＣＬ中ＨＡ含量随ＨＡＳＦ浓度升高呈剂量依赖性升高；ＦＰ－ＣＬ的最大收缩指数与其中ＨＡ含量呈正相关（ｒ＝０．９８７５，Ｐ＜０．０１）．结论：ＨＡＳＦ能促进三维培养的瘢痕成纤维细胞的收缩，这种作用可能是通过刺激其合成ＨＡ实现的．
Objective: To investigate the effect of hyaluronic acid stimulating factor (HASF) on the contractility of three-dimensional cultured scar fibroblasts. Methods: Purified rabbit-derived HASF was added to human dermal collagen-derived human hypertrophic scar fibroblasts three-dimensional culture system (FPCL). The contractile index of FPCL was calculated and the content of HA was determined by radioimmunoassay. Three-dimensional culture of scar fibroblasts shrinkage, rapid contraction and HA synthesis of the impact and its preliminary study. Results: HASF had a dose-effect relationship with the cell-mediated maximum contraction index of FPCL when the concentration of HASF was more than 1 × 10-3g / L in three-dimensional culture system. The difference between FPCL index and control group was significant P <0.01). HASF had no effect on the fast contraction of FPCL (P <0.05). The content of HA in FPCL increased with the increase of HASF in a dose-dependent manner. The maximum systolic index of FP-CL and the content of HA (R = 0.9875, P <0.01). Conclusion: HASF can promote the contraction of three-dimensional cultured scar fibroblasts. This effect may be achieved by stimulating the synthesis of HA.