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目的探讨膜联蛋白A7在人宫颈鳞癌和正常组织中的表达情况。方法应用免疫组织化学SP法,检测人宫颈鳞癌和正常宫颈组织蜡块各25例中膜联蛋白A7的表达差异;Western.blotting和半定量RT-PCR法检测人宫颈鳞癌和正常组织新鲜标本各25例中膜联蛋白A7的表达差异。结果膜联蛋白A7在组织中蛋白和mRNA水平表达一致,均为宫颈鳞癌组织表达高于正常宫颈组织,差异有统计学意义。结论膜联蛋白A7在人宫颈鳞癌表达上调,可能成为宫颈癌诊断的一个新的指示靶点。
Objective To investigate the expression of Annexin A7 in human cervical squamous cell carcinoma and normal tissues. Methods Immunohistochemical SP method was used to detect the expression of annexin A7 in 25 cases of cervical squamous cell carcinoma and normal cervical tissue. Western blotting and semi-quantitative RT-PCR were used to detect the expression of annexin A7 in cervical squamous cell carcinoma and normal tissues The 25 cases of specimens of Annexin A7 expression differences. Results Annexin A7 protein and mRNA levels in the expression of the same, both cervical squamous cell carcinoma tissue expression was higher than normal cervical tissue, the difference was statistically significant. Conclusions Annexin A7 is up-regulated in human cervical squamous cell carcinoma and may be a new target for diagnosis of cervical cancer.