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用30Gy、90Gy和180Gy 12C6+重离子辐照处理大葱干种子,研究了它对大葱的生物学效应。经过不同剂量12C6+重离子照射过的大葱,幼苗发芽率、株高等表型随着辐照剂量的增大,呈现明显的“抛物线”趋势。适量的12C6+重离子照射(30Gy)能提高大葱发芽率和抗旱、抗倒伏能力,促进生长发育。重离子辐照能有效地诱导大葱根尖细胞微核和染色体畸变形成,180Gy处理的大葱微核率和染色体畸变率最高达到9.09%和10.03%。本实验为大葱的重离子辐照育种打下基础。
The seeds of Welsh Onion were treated with 30Gy, 90Gy and 180Gy 12C6 + heavy ions and their biological effects on Welsh Onion were studied. After the different doses of 12C6 + heavy ion irradiated onion, seedling germination rate, plant height phenotype with the radiation dose increases, showing a clear “parabola ” trend. Appropriate amount of 12C6 + heavy ion irradiation (30Gy) can improve the germination rate of green onions and drought, lodging resistance, and promote growth and development. Heavy ion irradiation can effectively induce the formation of micronuclei and chromosome aberrations in root tip cells of Welsh Onion. The maximum rate of micronuclei and chromosome aberration of Welsh onion treated with 180Gy reached 9.09% and 10.03%. This experiment laid the foundation for heavy ion irradiation breeding of green onions.