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目的:研究五酯胶囊对他克莫司增效作用与ABCB1基因多态性的相关性研究。方法:通过聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定ABCB1 1236C>T(rs1128503)、ABCB1 2677G>T/A(rs2032582)和ABCB1 3435C>T(rs1045642)基因型,使用化学发光微粒子免疫分析技术(CMIA)检测他克莫司C_0。采用协方差分析考察不同组别患者他克莫司C_0/D的差异。结果:无论是单独使用他克莫司,还是他克莫司与五酯胶囊合用,ABCB1 1236C>T、ABCB1 2677G>T/A、ABCB1 3435C>T不同基因型及单倍型组的他克莫司C_0/D均无显著差异(P>0.05)。结论:五酯胶囊对他克莫司的增效作用与ABCB1 1236C>T、ABCB1 2677G>T/A、ABCB1 3435C>T基因多态性无关。
OBJECTIVE: To study the relationship between the effect of Wuzhi Capsule on the synergism of tacrolimus and the polymorphism of ABCB1 gene. Methods: The genotypes of ABCB1 1236C> T (rs1128503), ABCB1 2677G> T / A (rs2032582) and ABCB1 3435C> T (rs1045642) were determined by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) Tacrolimus C_0 was detected using chemiluminescence microparticle immunoassay (CMIA). Covariance analysis was used to investigate the difference of C_0 / D in tacrolimus in different groups. Results: Whether tacrolimus was used alone or tacrolimus combined with Wuzhi Capsule, tacrolimus of ABCB1 1236C> T, ABCB1 2677G> T / A, ABCB1 3435C> T genotypes and haplotypes Secretary C_0 / D were no significant difference (P> 0.05). Conclusion: The synergistic effect of Wuzhi capsule on tacrolimus has nothing to do with the polymorphisms of ABCB1 1236C> T, ABCB1 2677G> T / A and ABCB1 3435C> T.