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众多的研究表明,乙型肝炎病毒(HBV)感染及摄入黄曲霉毒素(AD是人类原发性肝癌(肝癌)的重要病因。测定人尿中黄曲毒毒素M_1(AFM_1)排出量,能灵敏、确切地反应Af摄入水平。本文对肝癌高发区部分乙型肝炎患者,进行了尿中AFM_1排出量检测,现将结果报告如下。对象:选居住肝癌高发区,血清乙型肝炎表面抗原(HBsAg)携带半年以上,血清谷丙转氨酶(ALT)异常,25～60岁的男性乙型肝炎患者为检测对象,共72例。尿样搜集:每月定期搜集被检对象晨尿1次,每1次100ml,混合保留在同一塑料桶内,存于-20℃冰柜中,6个月后进行测定。尿中AFM_1测定:(1)受检尿的前处理:取350ml混合尿,通过串接的G_2、G_3、G_4玻璃球过滤器,滤过的尿液以1.0～1.5ml/min速度通过抗-AFM-1层析小柱,而后用100％甲醇洗脱,搜集洗脱液作测定用。
Numerous studies have shown that hepatitis B virus (HBV) infection and the ingestion of aflatoxins (AD is an important cause of human primary liver cancer (HCC)). Determination of excretion of aflatoxin M_1 (AFM_1) Sensitive and accurately reflect the level of Af intake.This article on the part of high incidence of liver cancer in patients with hepatitis B, AFM_1 urinary excretion were detected, the results are reported as follows.OBJECTIVES: Select living high incidence of liver cancer, serum hepatitis B surface antigen (HBsAg) to carry more than six months, serum alanine aminotransferase (ALT) abnormalities, 25 to 60-year-old male patients with hepatitis B as the test object, a total of 72. Urine samples collected: monthly regular morning urine collected subjects were 1, Each time 100ml, mixed in the same plastic bucket, stored in -20 ℃ freezer, 6 months after the determination of urine AFM_1 determination: (1) urine test pretreatment: Take 350ml mixed urine through the string Then G_2, G_3, G_4 glass ball filters, filtered urine at 1.0 ~ 1.5ml / min speed through anti-AFM-1 chromatography column, and then eluted with 100% methanol, the eluent was collected for the determination use.