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目的探讨乙型肝炎患者血清乙肝病毒标志物(HBV-M)的临床意义。方法利用聚合酶链反应(PCR)技术对1000例血清样品进行HBV DNA定性检测,其中100份样品采用荧光标记(AmpliSensor)定量方法。结果定性PCR与免疫荧光定量PCR检测结果基本一致,在不同HBV-M模式中均可检出HBV DNA,其中HBsAg、HBeAg、抗-HBc阳性组HBV DNA检出率明显高于其他各组(P<0.01)。结论除HBsAg、HBeAg、抗-HBc阳性者外,其余HBV-M的各项表现形式中,均可存在不同程度的HBV复制,对HBV-M临床意义的认识需进行观念更新。
Objective To investigate the clinical significance of serum hepatitis B virus (HBV) M in patients with hepatitis B virus. Methods HBV DNA was detected qualitatively by polymerase chain reaction (PCR) in 1000 serum samples. 100 samples were detected by AmpliSensor quantitative method. Results The results of qualitative PCR and immunofluorescence quantitative PCR showed that HBV DNA was detected in different HBV-M patterns. The detection rate of HBV DNA in HBsAg, HBeAg and anti-HBc positive groups was significantly higher than that in other groups <0.01). Conclusions Except HBsAg, HBeAg and anti-HBc positive, HBV replication may exist in various manifestations of other HBV-M. The clinical significance of HBV-M needs to be updated.