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目的探讨胰腺肿瘤中核糖体S6蛋白激酶4(ribosomalS6 kinase 4,Rsk-4)表达状况及其与临床病理指标的可能关系。方法采用鼠抗人Rsk-4蛋白抗体和免疫组化EnVision两步法检测41例福尔马林固定、石蜡包埋的胰腺肿瘤(包括胰腺癌、实性假乳头性肿瘤、胰岛细胞瘤等)标本中Rsk-4表达状况并重点分析了其中32例胰腺癌表达水平与临床病理因素间的关系。结果胰腺癌组织中Rsk-4表达阳性率为37.5%(12/32),而癌旁正常胰腺组织Rsk-4表达阳性率为0(0/14),二者差异有显著性(P=0.009);Rsk-4的表达水平与肿瘤细胞分化程度相关(P=0.014),与患者性别、年龄、肿瘤大小、淋巴结转移、周围组织侵犯无关。在实性假乳头性肿瘤和胰岛细胞瘤中未见到Rsk-4表达。结论 Rsk-4蛋白在胰腺癌组织表达水平升高,提示Rsk-4可能在胰腺癌的发生和进展具有重要作用。
Objective To investigate the expression of ribosomal S6 kinase 4 (Rsk-4) and its possible relationship with clinicopathological parameters in pancreatic tumors. Methods Forty-one patients with formalin-fixed paraffin-embedded pancreatic cancer (including pancreatic cancer, solid pseudopapillary tumor, islet cell tumor, etc.) were detected by using mouse anti-human Rsk-4 protein antibody and immunohistochemistry EnVision two- The expression of Rsk-4 in the specimens and the relationship between the expression of 32 cases and the clinicopathological factors were analyzed. Results The positive rate of Rsk-4 in pancreatic cancer was 37.5% (12/32), while the positive rate of Rsk-4 in adjacent normal tissues was 0 (0/14), the difference was significant (P = 0.009 ). The expression level of Rsk-4 was correlated with the degree of tumor cell differentiation (P = 0.014), which was not related to the gender, age, tumor size, lymph node metastasis and surrounding tissue invasion. Rsk-4 expression was not seen in solid pseudopapillary tumors and islet cell tumors. Conclusion The expression of Rsk-4 protein in pancreatic cancer tissues is elevated, suggesting that Rsk-4 may play an important role in the development of pancreatic cancer.