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在许多生理及病理情况下,均可出现血清含醣基的结合蛋白质的增高,尤以肿瘤、炎症、结核等病变中最为显著,某些神经系统疾病患者血清及脑脊液中亦可能出现类似的变化。为了观察这种变化对中枢神经系统肿瘤的诊断价值,我们测定了30例健康者与67例中枢神经系统占位病变患者的血清醣蛋白及黏蛋白量。目前,关于含醣基的结合蛋白质的分类与命名,尚不统一,本文所用“血清醣蛋白”一辞系指血清中能被1.8M 过氯酸所沉淀的含醣基的结合蛋白质,而“黏蛋白”系指溶于过氯酸而不溶于磷钨酸的含醣基的结合蛋白质而言。测定方法采用 Weimer 与 Moshin 氏法(1953),将血清醣蛋白与粘蛋白分离,然后按 Shetlar氐等法(1948)分别测定此二类蛋白之己醣值。醣蛋白、黏蛋白量均以其所含之己醣(甘露糖+半乳糖)值来表示。
In many physiological and pathological conditions, there may be an increase in serum glycosyl-binding proteins, especially in tumors, inflammations, tuberculosis and other lesions. Similar changes may also occur in serum and cerebrospinal fluid in some patients with neurological diseases . To examine the diagnostic value of this change in CNS tumors, we measured serum glycoproteins and mucin levels in 30 healthy and 67 CNS patients. At present, the classification and nomenclature of glycosyl-containing binding proteins are not uniform. As used herein, the term “serum glycoprotein” refers to a glycosyl-containing binding protein in serum that can be precipitated by 1.8 M perchloric acid and “ Mucin ”refers to a glycosyl-containing binding protein that is soluble in perchloric acid and insoluble in phosphotungstic acid. Assay Methods Weimer and Moshin’s method (1953) were used to separate serum glycoproteins from mucins and then their hexose contents were measured by Shetlar’s method (1948). Glycoproteins, mucin content are all contained in its hexose (mannose + galactose) value to represent.