The stiffness matrix of semi-rigidly connected composite beams considering interface slip was established and the calculation method for elastic seismic response of composite frame was derived. The corresponding calculation programs were developed. Introducing the dimensionless quantities that were related to the connector shearing stiffness and the joint rotation stiffness, the influences of interface slip and semi-rigid joint on composite frame were transferred to quantitative parameter analysis, taking account of cross sectional properties, materials and linear stiffness of composite beam synthetically. Based on the calculation programs, free vibration frequencies and seismic responses of semi-rigid joint steel-concrete composite frame considering interface slip were calculated. The influences of interface slip and semi rigid joint on dynamic characteristics and seismic response were analyzed and the seismic design advices were presented. The results show that the interface slip decreases the free vibration frequencies and increase the seismic responses of composite frame. The semi-rigid joint reduces the free vibration frequencies and increases seismic responses of composite frame compared with rigid joint. With the increase of joint rotational stiffness, the elastic seismic responses of composite frame increase firstly and then decrease. The effects are related to the ratio of joint rotation stiffness to linear stiffness of composite beam.