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在研究中草药抗肿瘤有效成分时,如采用动物瘤谱进行筛选,常因工作最较大而遇到困难,特别是在有效成分提取分离过程中用以指示活性成分所在部位时,更会遇到工作量大,筛选周期较长的问题。为了寻找一种比较快速简易的筛选中草药抗肿瘤成分的方法,我们采用肉瘤180(以下简称S180)为瘤株的组织培养法,经试用认为具有筛选效果较好、简易快速、便于较大量筛选等优点。材料和方法一、培养基的组成:为了研究S180细胞的生长特性,共使用三种培养基进行对比。1、乳蛋白培养基:20%小牛血清,80%水解乳蛋白Hanks液(含水解乳蛋白0.5%);每100毫升中含生物素、胆硷、叶酸、烟酰胺、遍多酸钙、吡哆醛和硫胺各1毫克,对氨基苯甲酸和肌醇各2毫克,核黄素0.1毫克。
When studying the anti-tumor effective components of Chinese herbal medicines, if animal tumors are used for screening, they are often faced with the greatest difficulty because of their work, especially when they are used to indicate the active components in the active ingredient extraction and separation process. Large workload and long screening period. In order to find a relatively quick and easy method for screening anti-tumor components of Chinese herbal medicines, we used a tissue culture method in which sarcoma 180 (hereinafter abbreviated as S180) was used as a tumor strain. After trials, it was found that it has a better screening effect, is simple and rapid, and is convenient for large-scale screening. advantage. Materials and Methods 1. Composition of culture medium: In order to study the growth characteristics of S180 cells, a total of three culture media were used for comparison. 1. Milk protein medium: 20% calf serum, 80% hydrolyzed milk protein Hanks solution (containing 0.5% hydrolyzed milk protein); each 100 ml contains biotin, cholesterin, folic acid, nicotinamide, calcium polymenate, 1 mg each of pyridoxal and thiamine, 2 mg each of p-aminobenzoic acid and inositol, and 0.1 mg of riboflavin.