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目的利用正交设计优选复方石斛清肺散处方中药材的最佳工艺条件。方法以出膏率和橙皮苷含量为指标,采用HPLC法检测复方石斛清肺散中橙皮苷含量。并以L9(33)正交设计试验,对药材煎煮的水用量、煎煮次数和煎煮时间进行正交优选。结果最佳煎煮工艺为:10倍量水,浸泡35 min煎煮3次,每次煎煮1 h。方法学考察表明,橙皮苷在66.93~334.67 mg/L内线性关系良好;精密度试验、稳定性试验、重复性试验均良好。结论该含量测定方法与优选的煎煮工艺条件,操作简单,稳定,可靠,可为药品实际生产提供参考。
OBJECTIVE: To optimize the optimum conditions for the preparation of Chinese herbal medicines of Dendrobium Qingfei San by orthogonal design. Methods The rate of ointment and hesperidin were used as indexes to determine the content of hesperidin in compound dendrobium Qingfei San by HPLC. And L9 (33) orthogonal design test, the amount of boiling water medicine, boiling times and boiling time orthogonal optimization. Results The best decoction process: 10 times the amount of water, soaking 35 min boiling 3 times, each boiling 1 h. Methodological study showed that hesperidin in 66.93 ~ 334.67 mg / L within a good linear relationship; precision test, stability test, repeatability tests were good. Conclusion The determination of content and the preferred boiling process conditions, simple, stable, reliable, and can provide a reference for the actual production of drugs.