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目的:研究血浆病毒RNA拷贝数作为猴艾滋病（SAIDS）模型病毒学指标的必要性和重要性。方法:用SIVmac239静脉感染恒河猴,定期采血,血浆标本分别用血浆病毒分离法测定病毒水平,RT-PCR法测定血浆病毒RNA拷贝数。结果:52份标本中,除2份外,其余的标本凡是血浆病毒RNA拷贝数值>58.6×10～4cps/ml的,其血浆病毒分离结果为阳性;凡是病毒拷贝数值≤58.6×10～4cps/ml的,其血浆病毒分离结果为阴性,符合此规律的标本比例高达96.15％,且两种方法测出的血浆病毒变化趋势是一致的。结论:血浆病毒RNA拷贝数是猴艾滋病模型不可缺少的病毒学指标。
Objective: To study the necessity and importance of RNA copy number of plasma virus as a virological indicator of Monkey AIDS (SAIDS) model. Methods: Rhesus monkeys were infected intravenously with SIVmac239, and the blood samples were collected periodically. The virus levels were determined by plasma viral isolation and the copy number of plasma viral RNA by RT-PCR. Results: Of the 52 specimens, all the other specimens were positive for plasma viral RNA copy number, the copy number of the virus was less than or equal to 58.6 × 10 ~ 4cps / ml, ml, the results of its plasma virus isolation was negative, the proportion of specimens in line with this law up to 96.15%, and the trend of the two viruses measured by the two methods is consistent. Conclusion: The RNA copy number of plasma virus is an indispensable virological indicator of monkey AIDS model.