论文部分内容阅读
目的应用高速逆流色谱法分离制备木香中的木香烃内酯和去氢木香内酯。方法应用正己烷-乙酸乙酯-甲醇-水(2:0.5:2:1)为两相溶剂系统,主机转速为850 r.m in-1,流速为2.0 m l.m in-1,检测波长为254 nm。结果从100 mg木香粗提物中分离得到34.6 mg木香烃内酯、44.3 mg去氢木香内酯,所得产物经HPLC检测纯度均大于98%。结论所用方法分离制备木香烃内酯和去氢木香内酯具有简便、快速的优点。
OBJECTIVE: To isolate and prepare woody lactone and dehydrocostus lactone in woody wood by high-speed countercurrent chromatography. The method used n-hexane-ethyl acetate-methanol-water (2:0.5:2:1) as the two-phase solvent system, the host speed was 850 rm in-1, the flow rate was 2.0 mlm in-1, and the detection wavelength was 254 nm. . Results 34.6 mg of dehydrocostus lactone and 44.3 mg of dehydrocostus lactone were isolated from the crude extract of 100 mg of woody incense. The purity of the obtained product was more than 98% by HPLC. Conclusion The method used for the separation and preparation of costolactone and dehydrocostuslactone is simple and rapid.