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目的通过研究SLE患者T淋巴细胞CD70mRNA及其蛋白的表达,探讨CD70在SLE发病机理中的作用。方法用定量RT-PCR方法测定15例活动期、15例非活动期SLE患者和15例正常人对照组外周血T淋巴细胞CD70mRNA转录水平。用流式细胞仪检测各组的CD70+CD4+T淋巴细胞阳性率。结果活动期、非活动期SLE患者及正常对照组T淋巴细胞CD70mRNA转录水平分别为(0.82±0.12),(0.73±0.11)和(0.45±0.09),活动期、非活动期SLE患者明显高于正常对照组,差异有统计学意义(P<0.01),且活动期显著高于非活动期SLE患者,差异有统计学意义(P<0.01)。活动期、非活动期SLE患者及正常人外周血CD4+CD70+T淋巴细胞阳性率分别为(80.30±11.04)%,(66.80±3.98)%和(12.48±3.45)%,活动期、非活动期SLE患者明显高于正常对照组,差异有统计学意义(P<0.01),且活动期显著高于非活动期SLE患者,差异有统计学意义(P<0.01)。结论 CD70的过度表达在SLE的发生发展中起着重要的作用,并且CD70过度表达可以作为SLE疾病活动的一个指标。
Objective To investigate the role of CD70 in the pathogenesis of SLE by studying the expression of CD70mRNA and its protein in T lymphocytes from patients with SLE. Methods The transcription level of CD70mRNA in peripheral T lymphocytes from 15 patients with active SLE and 15 normal controls were measured by quantitative RT-PCR. The positive rate of CD70 + CD4 + T lymphocytes in each group was detected by flow cytometry. Results The levels of CD70mRNA in T lymphocytes in active and inactive SLE patients and normal controls were (0.82 ± 0.12) and (0.73 ± 0.11) and (0.45 ± 0.09), respectively. The active and inactive SLE patients were significantly higher than those in inactive SLE patients The difference between the two groups was statistically significant (P <0.01), and the activity was significantly higher than inactive SLE patients, the difference was statistically significant (P <0.01). The positive rates of CD4 + CD70 + T lymphocytes in active and inactive SLE patients and normal persons were (80.30 ± 11.04)%, (66.80 ± 3.98)% and (12.48 ± 3.45)%, respectively, The SLE patients were significantly higher than the normal control group, the difference was statistically significant (P <0.01), and the activity was significantly higher than inactive SLE patients, the difference was statistically significant (P <0.01). Conclusion Overexpression of CD70 plays an important role in the development and progression of SLE. CD70 overexpression can be used as an indicator of SLE disease activity.