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体外细胞微核试验,作为一种鉴定化学物致突变性的体外试验已广泛应用。常规采用的制片方法是在细胞收获后,先作低渗处理,然后涂片、固定和染色.由于细胞经受试化学物处理后,细胞膜对低渗的耐受性常常会发生变化,使得低渗处理不易掌握,易造成低渗不够或过头。我们采用美国制造的离心制片机(Cytaspin Shandon Inc.pittsb-urgh,PA15275),在细胞收获后,将细胞混悬液调整至适当的细胞密度,不经低渗直接制片,胞
In vitro cellular micronucleus assays have been widely used as in vitro assays to identify mutagenicity of chemicals. Conventional methods of preparation are hypotonic treatment followed by smear, fixation, and staining after harvesting of the cells Since the cell membrane undergoes a subtle effect on hypotonicity after treatment with the test chemicals, the low Infiltration is not easy to grasp, easily lead to inadequate or excessive hypotonic. Using Cytaspin Shandon Inc. patents (PA15275) manufactured in the USA, we adjusted the cell suspension to the appropriate cell density after harvesting the cells, directly producing the cells without hypotonicity