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从受体水平探讨锂盐改善胰岛素敏感性的作用机制。方法 :红细胞经RPMI - 16 40孵育 30min ;用对甲苯磺酰氟 (PMSF)及超速低温离心等方法制备红细胞胰岛素受体标本 ;按Haruhiko酶联免疫吸附法(ELISA)分析红细胞磷酸化胰岛素受体数。结果 :胰岛素预处理Ⅱ型糖尿病患者红细胞后的磷酸化胰岛素受体数较对照组减少 ;用氯化锂预处理Ⅱ型糖尿病患者及对照组红细胞吸光度分别为 (1mM氯化锂 ) :0 .439±0 .12 1、0 .399± 0 .0 98;(10mM氯化锂 ) :0 .5 14± 0 .130、0 .384± 0 .10 2 ,表明 1～ 10mM氯化锂能引起二者红细胞胰岛素受体磷酸化 (P <0 .0 1) ,前者的反应显著高于后者 ;经 10 -7M胰岛素加 1mM氯化锂联合处理的Ⅱ型糖尿病患者的红细胞 ,其磷酸化胰岛素受体数高于单独用 10 -7M胰岛素者。结论 :肥胖的Ⅱ型糖尿病患者存在胰岛素受体水平 (酪氨酸激酶活性降低 )抵抗 ;氯化锂能导致Ⅱ型糖尿病患者及健康人红细胞胰岛素受体磷酸化 ,推测锂能激活酪氨酸激酶而发挥胰岛素样作用 ;在胰岛素存在的情况下加用锂盐对改善Ⅱ型糖尿患者胰岛素受体水平敏感性有协同作用
From the receptor level to explore the mechanism of lithium salt to improve insulin sensitivity. Methods: Erythrocytes were incubated with RPMI - 1640 for 30 min. PMSF and ultracentrifuge were used to prepare erythrocyte insulin receptor. The erythrocyte phosphorylated insulin receptor was analyzed by Haruhiko enzyme - linked immunosorbent assay (ELISA) number. Results: The number of phosphorylated insulin receptor in erythrocytes of type 2 diabetic patients pretreated with insulin decreased compared with control group. The absorbance of erythrocytes of type 2 diabetic patients pretreated with lithium chloride and control group were (1mM lithium chloride): 0.439 ± 0.12 1,0 .399 ± 0.098; (10mM lithium chloride): 0.514 ± 0.13O, 0.384 ± 0.10 2, indicating that 1 ~ 10mM lithium chloride can cause two Erythrocyte insulin receptor phosphorylation (P <0.01), the former response was significantly higher than the latter; the 10 -7M insulin plus 1mM lithium combined treatment of type II diabetes red blood cells, its phosphorylated insulin receptor The body mass is higher than 10 -7M insulin alone. CONCLUSION: Obese type 2 diabetic patients have insulin receptor level (tyrosine kinase activity decreased) resistance; lithium chloride can lead to type II diabetes and healthy human erythrocyte insulin receptor phosphorylation, presumably lithium can activate tyrosine kinase And play an insulin-like role; in the presence of insulin plus lithium salt to improve the sensitivity of type 2 diabetes patients insulin receptor has a synergistic effect