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目的评价不同引物检测庚型肝炎病毒（HGV）的效果和了解其与丙型肝炎病毒（HCV）混台感染率及相互关系．方法5’-NCR，NSS引物和PCRELISA检测HGV，定量RT－PCR检测HCV及部分标本中的HCV分型，部分扩增产物测定核苷酸序列。结果211例HCV阳性血清标本中HGV5－NCRRNA和HGVNSSRNA检出率分别为47．9％和31．8％，总阳性率高达49．8％。结论HGV和HCV有较高的混合感染率。PCRELISA是一种极为敏感的HGV检测方法，HGV5’－NCR引物的检测效果优于HGVNS5。核苷酸序列分析证实PCR—ELISA检测结果可靠。标本中HCV浓度与HGV感染呈负相关，可能存在相互抑制的机制。
Objective To evaluate the efficacy of different primers in detecting Hepatitis G virus (HGV) and to understand its relationship with Hepatitis C virus (HCV). Methods 5’-NCR, NSS primers and PCRELISA were used to detect HGV. Quantitative RT-PCR was used to detect the HCV genotypes in HCV and some samples. The nucleotide sequences of some of the amplified products were determined. Results The positive rates of HGV5-NCRRNA and HGVNSSRNA in 211 HCV positive serum samples were 47.9% and 31.8%, respectively, with a total positive rate of 49.8%. Conclusion HGV and HCV have a higher mixed infection rate. PCRELISA is a very sensitive HGV detection method, HGV5’-NCR primer detection better than HGVNS5. Nucleotide sequence analysis confirmed the PCR-ELISA test results are reliable. There is a negative correlation between HCV concentration and HGV infection in the specimens, and there may be a mechanism of mutual inhibition.