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目的:建立胆康胶囊中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量测定方法。方法:采用Sapphire C18色谱柱(4.6×150mm,5μm),流动相为甲醇-0.1%磷酸溶液(75:25),流速1.0ml/min,检测波长为254nm。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的进样量分别在0.011878～0.111878μg(r=0.9999),0.01308～0.1308μg(r=0.9998),0.01338～0.1338μg(r=0.9997),0.05151～0.5151μg(r=0.9999),0.015229～0.15229μg(r=0.9999)范围内与峰面积成良好的线性关系,平均加样回收率分别为98.25%(RSD=0.12%),101.09%(RSD=0.28%),99.17%(RSD=0.18%),97.07%(RSD=0.51%),101.33%(RSD=0.64%)。结论:本方法简便、准确、重现性好,可用于胆康胶囊的质量控制。
Objective: To establish a method for the determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in biliary capsule. Methods: Sapphire C18 column (4.6 × 150 mm, 5 μm) was used. The mobile phase was methanol-0.1% phosphoric acid solution (75:25). The flow rate was 1.0 ml / min and the detection wavelength was 254 nm. Results: The injection volume of aloe-emodin, rhein, emodin, chrysophanol and physcion were 0.011878 ~ 0.111878μg (r = 0.9999), 0.01308 ~ 0.1308μg (r = 0.9998), 0.01338 ~ 0.1338μg (r = 0.9997), 0.05151 ~ 0.5151μg (r = 0.9999) and 0.015229 ~ 0.15229μg (r = 0.9999). The average recoveries were 98.25% (RSD = 0.12% , 101.09% (RSD = 0.28%), 99.17% (RSD = 0.18%), 97.07% (RSD = 0.51%) and 101.33% (RSD = 0.64%). Conclusion: The method is simple, accurate, reproducible, and can be used for quality control of biliary capsule.