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目的探讨染矽尘大鼠血、尿中硅含量与肺组织中羟脯氨酸、Ⅰ型胶原、Ⅲ型胶原含量的关系。方法处理组采用气管灌注法注入矽尘混悬液建立大鼠矽肺模型,对照组采用同样处理但用生理盐水代替矽尘混悬液。两组取第28天大鼠的血液、尿液,经样品前处理后用原子吸收分光光度计检测;大鼠肺组织制作石蜡切片,采用天狼猩红染色法,用偏振光显微镜和图像分析系统对肺组织Ⅰ型胶原、Ⅲ型胶原的表达进行分析;试剂盒检测肺组织羟脯氨酸含量。结果处理组大鼠血硅、尿硅、Ⅰ型胶原及Ⅲ型胶原明显高于对照组(P<0.05),羟脯氨酸虽稍低于对照组,但无统计学意义(P>0.05);血硅、尿硅分别与Ⅰ型胶原、Ⅲ型胶原建立直线回归,有统计学意义(P<0.05),血硅、尿硅分别与羟脯氨酸建立直线回归,无统计学意义(P>0.05)。结论血硅、尿硅对早期矽肺纤维化程度可能有一定的指示意义。
Objective To investigate the relationship between silicon content in blood and urine of rats exposed to silica dust and the content of hydroxyproline, collagen Ⅰ and collagen Ⅲ in lung tissues. Methods The rats in the treatment group were injected with silica dust suspension into the silicosis model by tracheal perfusion. The control group was treated with the same treatment but replaced with silicalite suspension with saline. Two groups of blood and urine were taken from the rats on the 28th day. The samples were pretreated with an atomic absorption spectrophotometer. The lung tissues of the rats were made into paraffin sections. The cells were analyzed by Sirius Red staining and analyzed by polarized light microscope and image analysis system The expression of type I collagen and type III collagen in lung tissue was analyzed. The content of hydroxyproline in lung tissue was detected by kit. Results Serum silicon, urine silicon, type I collagen and type Ⅲ collagen in the treatment group were significantly higher than those in the control group (P <0.05), while hydroxyproline was slightly lower than the control group (P> 0.05) (P <0.05). There was a linear regression between blood silicon and urinary silicon and type Ⅰ collagen and type Ⅲ collagen, respectively (P <0.05), and there was no significant difference between blood silicon and urinary silicon and hydroxyproline > 0.05). Conclusion Blood silicon and urine silicon may have some indication to the degree of early silicosis.