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目的探讨供体活细胞输注对移植物慢性排斥反应的影响及长期存活的移植物中VEGF、ET-1的表达。方法建立Wistar→SD大鼠心脏移植排斥反应模型,于术中经门静脉注射供体活细胞(脾细胞、骨髓细胞、肝细胞)诱导特异性免疫耐受,观察生存时间,对长期存活移植物进行病理形态学分析及半定量逆转录聚合酶链反应(RT-PCR)检测。结果同时注射两种或两种以上供体活细胞可使移植物长期存活(>90d)。长期存活的移植物血管病变明显减轻,仅有轻度炎症细胞浸润和间质纤维化,与CsA组及单一细胞注射组相比差异具有显著性意义(P<0.05)。联合注射组VEGF、ET-1表达明显减低。结论经门静脉注射两种或两种以上供体活细胞可减轻慢性排斥反应,VEGF、ET-1的表达与血管硬化及纤维化程度呈正相关。
Objective To investigate the effects of donor live cell infusion on chronic allograft rejection and the expression of VEGF and ET-1 in long-term graft. Methods The Wistar → SD rat model of cardiac allograft rejection was established. Specific immune tolerance was induced by intravenous injection of living donor cells (spleen cells, bone marrow cells and hepatocytes) during operation. The survival time was observed and the long-term survival graft Pathomorphology analysis and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) detection. Results Simultaneous injection of two or more living donor cells resulted in long-term graft survival (> 90 days). Long-term survival of graft vascular lesion was significantly reduced, only mild inflammatory cell infiltration and interstitial fibrosis, compared with CsA group and single cell injection group, the difference was significant (P <0.05). The combined injection group VEGF, ET-1 expression was significantly reduced. Conclusion Intravenous injection of two or more live donor cells can reduce chronic rejection. The expressions of VEGF and ET-1 are positively correlated with the degree of vascular sclerosis and fibrosis.