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目的从IL-4/STAT6信号通路入手,以该通路负反馈调节因子SOCS1和关键因子STAT6为检测指标,多角度探查溃疡性结肠炎的发生与IL-4的关系。方法将实验动物分为6组,采用免疫法造模,用免疫组化法检测大鼠SOCS1、STAT6的活性。结果模型组实验大鼠SOCS1活性(0.485 5±0.178 0)显著低于正常组(0.655 2±0.187 4),P<0.05;而STAT6活性(0.803 0±0.208 2)则明显高于正常组(0.589 3±0.191 9),P<0.05。四逆散能显著增加实验大鼠SOCS1活性(0.576 5±0.208 0)(P<0.05),降低STAT6活性(0.579 8±0.152 6)(P<0.05);柴芍配伍(0.562 5±0.275 7)对SOCS1活性的影响与四逆散(0.576 5±0.208 0)比较差异无统计学意义,对STAT6活性(0.529 4±0.144 3)表达的影响显著优于四逆散(0.579 8±0.152 6)(P<0.05);芍枳甘配伍(0.616 9±0.200 6)对STAT6活性的影响作用明显,与四逆散(0.579 8±0.152 6)比较差异无统计学意义;柴枳甘配伍对SOCS1(0.509 1±0.227 9)、STAT6(0.756 1±0.263 6)的影响与模型组(0.485 5±0.178 0;0.803 0±0.208 2)比较差异无统计学意义。结论四逆散能够通过刺激SOCS1活性,抑制STAT6活性表达来调节IL-4/STAT6通路,进而干预实验性溃疡性结肠炎。其中,柴芍配伍作用明显;芍枳甘配伍对STAT6活性的影响作用明显,但对SOCS1的影响不显著;柴枳甘配伍对SOCS1、STAT6的影响均不显著。
OBJECTIVE: To investigate the relationship between the occurrence of ulcerative colitis and IL-4 in a multi-angle way from the IL-4 / STAT6 signaling pathway and SOCS1, a negative feedback regulator of this pathway, and STAT6, a key factor. Methods The experimental animals were divided into 6 groups. The immune system was used to establish the model. The activities of SOCS1 and STAT6 in rats were detected by immunohistochemistry. Results The activity of SOCS1 in experimental group (0.485 5 ± 0.178 0) was significantly lower than that in normal group (0.655 2 ± 0.187 4, P <0.05), and STAT6 activity was significantly higher than that in normal group 3 ± 0.191 9), P <0.05. Sini Powder can significantly increase SOCS1 activity (0.576 5 ± 0.208 0) (P <0.05) and decrease STAT6 activity (0.579 8 ± 0.152 6) in experimental rats (P <0.05) The effect on SOCS1 activity was not significantly different from that of Sini Powder (0.576 5 ± 0.208 0), and the effect on the activity of STAT6 (0.529 4 ± 0.144 3) was significantly better than that of Sini Powder (0.579 8 ± 0.152 6) P <0.05). Combination of Shao Zhi Huang (0.616 9 ± 0.200 6) had a significant effect on the activity of STAT6, but there was no significant difference with that of Sini San (0.579 8 ± 0.152 6) 1 ± 0.227 9), and STAT6 (0.756 1 ± 0.263 6) had no significant difference with the model group (0.485 5 ± 0.178 0; 0.803 0 ± 0.208 2). Conclusion Sini Decoction can regulate IL-4 / STAT6 pathway by stimulating SOCS1 activity and inhibiting the expression of STAT6, thereby intervening experimental ulcerative colitis. Among them, the effect of compatibility of Chaihao was obvious. The effect of compatibility of Shaozhigan and Ganoderma lucidum on STAT6 activity was obvious, but the effect on SOCS1 was not significant. The effect of Chairan and Gangen on SOCS1 and STAT6 was not significant.