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以披针叶黄华试管苗为材料,研究不同质量分数的NaCl和Na2SO4胁迫对其生理生化指标的影响。结果显示,披针叶黄华试管苗鲜重、干重、单株叶片数及叶绿素含量在0.2%Na2SO4胁迫下均高于对照组,而在0.4%~1.0%Na2SO4和0.2%~1.0%NaCl胁迫下,试管苗鲜重、干重、平均株高、单株叶片数及叶绿素含量均随盐胁迫浓度的增加而下降,且与对照组比较差异显著,与NaCl相比,Na2SO4胁迫下试管苗生长指标和叶绿素含量的变化均小于相同浓度的NaCl胁迫;试管苗叶片的超氧化物歧化酶(SOD)活性在NaCl胁迫下随着盐质量分数的增加呈先上升后下降的趋势,在Na2SO4胁迫下呈下降-上升-下降的变化趋势,而过氧化氢酶(CAT)和过氧化物酶(POD)活性均随着2种盐质量分数的增加呈先下降后上升的趋势。研究发现,披针叶黄华试管苗通过提高SOD、CAT以及POD活性,清除盐胁迫下产生的过量活性氧,保护膜结构,缓解盐伤害,同时,披针叶黄华试管苗适应Na2SO4胁迫的能力强于NaCl。
In order to study the effects of different NaCl and Na2SO4 stress on its physiological and biochemical parameters, The results showed that under the stress of 0.2% Na2SO4, the fresh weight, dry weight, number of leaves per plant and chlorophyll content of test tube plantlets were higher than those of the control group. Under the stress of 0.4% -1.0% Na2SO4 and 0.2% -1.0% NaCl stress, , Fresh weights of plantlets, dry weight, average plant height, number of leaves per plant and chlorophyll content all decreased with the increase of salt stress concentration, compared with the control group, the difference was significant. Under NaCl stress, And chlorophyll contents were all lower than those under the same concentration of NaCl stress. The activity of superoxide dismutase (SOD) in test-tube seedlings increased firstly and then decreased with the increase of salt content under NaCl stress. Under Na2SO4 stress (CAT) and peroxidase (POD) activity both decreased first and then increased with the increase of the two salt contents. The results showed that in the test-tube plantlets, the activity of SOD, CAT and POD were increased, and the excessive reactive oxygen species produced by salt stress were removed to protect the membrane structure and alleviate salt damage. At the same time, .