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作者用细胞培养的方法,检测恶性淋巴瘤(ML)患者的淋巴结,周围血,骨髓标本中是否有能形成克隆并增殖的肿瘤干细胞群体及与预后的关系。血及骨髓分离得单个核细胞悬液,淋巴结制成单细胞悬液。半固体培养:体系包括Iscove’s培养液,30%少血小板的正常人血浆,5×10~(-5)mol/L二巯基己醇(2ME),每个标本做两种培养,加和不加
The authors used cell culture method to detect lymph node, peripheral blood and bone marrow specimens from patients with malignant lymphoma (ML) for the presence or absence of clonal and proliferating cancer stem cell populations and its relationship with prognosis. Blood and bone marrow isolated mononuclear cell suspension, lymph node made single cell suspension. Semi-solid culture: The system includes Iscove’s broth, normal human plasma with 30% platelet, and 5 × 10 ~ (-5) mol / L dimercaptohexanol (2ME)