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本研究采用Illumina Hi Seq 2500技术对地黄脱毒苗进行转录组测序,获得了地黄转录组信息,并对构建好的地黄基因Unigene库进行蛋白功能注释、KEGG代谢通路分析等生物信息学分析,以期大规模挖掘与地黄环烯醚萜类成分生物合成及后修饰相关的基因。应用高通量测序技术对地黄组培苗进行转录组测序,得到13.61Gb的干净数据,De novo组装后共获得70778条Unigene,其中33.428(47.23%)条unigenes能被Nr、Swiss-Prot、Pfam、KOG、KEGG、GO和COG等公共数据库注释。进一步对被注释的地黄萜类生物合成及后修饰相关基因进行挖掘发现,地黄转录组中共有81个Unigene参与萜类骨架合成;共有226个Unigene可能参与CYP450介导的环烯醚萜骨架的氧化修饰;共有102个Unigene与糖基转移酶相关。地黄转录组数据库的获得为探索地黄环烯醚萜类成分的生物合成研究奠定了基础,为后续基因功能的分析等研究提供分子水平依据。
In this study, Illumina Hi Seq 2500 technology was used to transcribe transcriptome of Rehmannia glutinosa Libosch and obtain the information of Radix Rehmanniae transcriptome. Bioinformatic analysis was performed on the constructed Unigenes gene library of Rehmannia glutinosa, KEGG metabolic pathway analysis, Large-scale excavation and Rehmannia iridoids biosynthesis and post-modification related genes. High throughput sequencing of transcriptome of Rehmannia glutinosa seedlings was performed to obtain clean data of 13.61Gb. 70778 Unigene were obtained after De novo assembly, of which 33.428 (47.23%) unigenes could be detected by Nr, Swiss-Prot, Pfam , KOG, KEGG, GO and COG public database annotations. Further digging the annotated genes related to biosynthesis and post-modification of Rehmannia glutinosae, we found that 81 Unigenes of Rehmannia glutinosa transcriptome are involved in terpenoid skeleton synthesis; a total of 226 Unigene may be involved in the CYP450-mediated oxidation of iridoid skeleton Modified; a total of 102 Unigene related glycosyltransferases. The obtainment of Rehmannia glutinosa transcriptome database lays the foundation for the research on the biosynthesis of terpenoid constituents of Rehmannia glutinosa and provides molecular level basis for the subsequent analysis of gene function.