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单染色体片段代换系是QTL精细定位的理想材料。水稻对细菌性条斑病的抗性属于典型的数量抗性。本研究以课题组构建的携有多个细条病抗性QTL(qBlsr3d,qBlsr5a和qBlsr5b)的近等基因系H359R为供体亲本,以感病品种H359为受体亲本,通过杂交,多次回交和自交,并结合分子标记辅助选择,成功构建了仅携有QTL qBlsr3d的单染色体片段代换系,命名为H359-BLSR3D。进一步通过分子标记定位分析表明:渗入区段位于第3号染色体分子标记3DSSR3和3DSSR12之间,物理距离约为1 250 kb。田间接种鉴定表明:H359-BLSR3D与感病亲本H359间的细条病抗性差异显著,结果证明了抗性QTL qBlsr3d的真实存在。H359-BLSR3D将为QTL qBlsr3d的精细定位和最终克隆奠定基础。
Single chromosome fragment substitution lines are ideal materials for the fine mapping of QTLs. Resistance of rice to bacterial leaf streak is a classic quantitative resistance. In this study, H359R, a near-isogenic line with multiple stripe disease resistance QTLs (qBlsr3d, qBlsr5a and qBlsr5b), was constructed by the research group as the donor parent. The susceptible parent H359 And selfing, and combined with molecular marker-assisted selection, we successfully constructed a single chromosomal segment substitution line carrying qBlsr3d QTL, named as H359-BLSR3D. Further analysis by molecular marker localization showed that the infiltration zone was located between chromosome 3 3DSSR3 and 3DSSR12 with a physical distance of about 1 250 kb. The field inoculation identification showed that there was a significant difference in the resistance to sliver disease between H359-BLSR3D and the susceptible parent H359, which demonstrated the true existence of the resistant QTL qBlsr3d. H359-BLSR3D will lay the foundation for the fine mapping and final cloning of QTL qBlsr3d.