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慢性分泌性中耳炎反复发作原因尚不甚清楚。近年来免疫学方面的研究越来越多。本文观察了30例1～11岁(平均4.5±2.6岁)反复发作分泌性中耳炎儿童的血清和中耳渗出液中的C3裂解产物和C3裂解活性,其方法为:30例(36耳)患儿均在全麻下采血和抽吸中耳渗出液,标本加等量生理盐水,其中10例血清和中耳渗出液中加入0.01M的乙二胺四乙酸(EDTA),于6小时内放于-70℃冻存。两周内第一次解冻,进行补体成份、C 3转化和C3d含量测定;两个月
The causes of recurrent chronic otitis media is not yet clear. In recent years, more and more immunology research. In this study, C3 lysates and C3 cleavage activities in serum and middle exudate of 30 children aged 1 to 11 years (average 4.5 ± 2.6 years) with recurrent otitis media with effusion were measured in 30 patients (36 ears) Children under general anesthesia blood and aspirate the middle ear exudate, specimens plus equal amount of saline, of which 10 cases of serum and middle ear exudate by adding 0.01M of ethylenediaminetetraacetic acid (EDTA), at 6 Put it at -70 ℃ in cold storage for hours. First thaw within two weeks, complement composition, C3 conversion and C3d determination; two months