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目的:探讨HMGB1在a2A-肾上腺素受体介导脓毒血症大鼠急性肺损伤(ALI)的作用。方法:64大鼠建立盲肠结扎穿孔法(CLP)脓毒症模型,随机均分为以下两组:CLP组及CLP+马来酸钠组。各组分别于模型建立后2(T1)、6(T2)、12(T3)、24 h(T4)时检测大鼠血清TNF-、高迁移率族蛋白1(HMGB1)及IL-10含量。CLP24 h后检测肺组织干湿重比(W/D)和髓过氧化物酶活性(MPO)及HMGB1表达;并采用HE法进行肺组织学评分。结果:CLP+马来酸钠组T2时的TNF-水平明显低于CLP组(P<0.05);而HMGB1在T2、T3及T4均明显低于CLP组(P<0.05);IL-10在各个时间点比较结果差异无统计学意义(P>0.05)。CLP+马来酸钠组肺组织W/D、MPO活性、肺组织损伤评分均明显低于CLP组(P<0.05)。CLP+马来酸钠组肺组织HMGB1表达明显低于CLP组(P<0.05)。结论:HMGB1参与了ALI的病理过程,a2A-肾上腺素受体阻断可以通过抑制HMGB1从而改善ALI时的肺功能。
AIM: To investigate the role of HMGB1 in a2A-adrenergic receptor-mediated acute lung injury (ALI) in septic rats. Methods: Sixty-four rats were established cecal ligation and puncture (CLP) sepsis model and randomly divided into the following two groups: CLP group and CLP + sodium maleate group. The serum levels of TNF-, HMGB1 and IL-10 in rats in each group were measured at 2 (T1), 6 (T2), 12 (T3) and 24 h (T4) After 24 h CLP, the W / D and MPO and HMGB1 expression in lung tissue were measured. The lung histological score was determined by HE method. Results: The levels of TNF-α in CLP + sodium maleate group at T2 were significantly lower than those in CLP group (P <0.05), while HMGB1 at T2, T3 and T4 were significantly lower than those in CLP group (P <0.05) There was no significant difference between the time points (P> 0.05). The lung W / D, MPO activity and lung injury scores in CLP + sodium maleate group were significantly lower than those in CLP group (P <0.05). The expression of HMGB1 in CLP + sodium maleate group was significantly lower than that in CLP group (P <0.05). CONCLUSIONS: HMGB1 is involved in the pathological process of ALI, and a2A-adrenergic receptor blockade may improve lung function at ALI by inhibiting HMGB1.