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目的:检测人食管鳞状细胞癌TE-13细胞中线粒体DNA(mtDNA)与细胞凋亡的发生情况及可能的关系。方法:TE-13细胞分为2组:溴化乙啶(EB)处理组在细胞培养液中加入50mg/LEB,未处理组常规培养;在培养第4、8和12天分别利用半定量RT-PCR检测2组细胞mtDNA的表达,利用流式细胞术检测细胞的凋亡情况。结果:EB处理组的第4天和第8天mtDNA的表达量分别为(0.467±0.031)和(0.197±0.015),到第12天mtDNA已完全丢失,未处理组分别为(0.660±0.046)、(0.673±0.031)和(0.653±0.021),差异有统计学意义(F组间=1133.878,F时间=109.058,F交互=104.597;P<0.001)。流式细胞术检测结果显示,EB处理组凋亡细胞百分比高于未处理组(F组间=2773.035,F时间=407.652,F交互=406.641;P<0.001)。结论:抑制TE-13细胞中mtDNA的复制可能会促进细胞凋亡。
Objective: To detect the occurrence of mitochondrial DNA (mtDNA) in human esophageal squamous cell carcinoma TE-13 cells and its possible relationship with cell apoptosis. Methods: The TE-13 cells were divided into two groups: 50 mg / LEB was added to the cell culture medium in the ethidium bromide (EB) group, and the untreated group was routinely cultured. On the 4th, 8th and 12th day, The expression of mtDNA in two groups of cells was detected by PCR, and the apoptosis of cells was detected by flow cytometry. Results: The expression of mtDNA in EB group was (0.467 ± 0.031) and (0.197 ± 0.015) on the 4th and 8th day, respectively, and the mtDNA was completely lost on the 12th day in the EB group (0.660 ± 0.046) , (0.673 ± 0.031) and (0.653 ± 0.021), respectively. The difference was statistically significant (F group = 1133.878, F time = 109.058, F interaction = 104.597; P <0.001). Flow cytometry results showed that the percentage of apoptotic cells in EB-treated group was higher than that in untreated group (F = 2773.035, F = 407.652, F = 406.641; P <0.001). Conclusion: Inhibition of mtDNA replication in TE-13 cells may promote apoptosis.