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Ca761/L是将615系小鼠实体型乳腺癌株(Ca761/S)在体外培养而建成的一个小鼠乳腺癌细胞系,它是一个生长迅速而稳定的悬浮培养的瘤细胞系,迄今已在体外培养二年,传至150余代。其细胞形态以中圆形为主,多为单核,电镜下显示恶性细胞的征象并可见到A型病毒样颗粒。细胞化学(DNA、RNA、SDH、5′AMPase、ACP等)的观察结果与电镜所见相符。培养至第5天细胞可增长27.4倍。分裂指数以第3天最高达18‰。瘤细胞核型为45或43,-2X,tri12±tri17,+M1～5。≥7μg/ml ConA就产生凝集反应,甚至不加ConA也可能出现自发凝集。将5×10~6浓度细胞经不同途径接种于615系小鼠,成瘤率达100%。无一例自发缓解且保存其转移特性。支原体检测阴性。因此,Ca761/L具有较大的推广应用价值。
Ca761/L is a mouse breast cancer cell line constructed by in vitro culture of a 615-type mouse solid breast cancer strain (Ca761/S). It is a rapidly growing and stable suspension cultured tumor cell line. In vitro culture for two years, spread to more than 150 generations. Its cell morphology is mainly round and mostly mononuclear. Electron microscope shows the signs of malignant cells and A type virus-like particles can be seen. Observations of cellular chemistry (DNA, RNA, SDH, 5′AMPase, ACP, etc.) were consistent with the findings of electron microscopy. By the fifth day of culture, the cells can grow 27.4 times. The split index reached 18 in the third day. The tumor cell karyotype was 45 or 43, -2X, tri12 ± tri17, +M1 ~ 5. ≥7μg/ml ConA produces an agglutination reaction, and spontaneous agglutination may occur even without ConA. The 5×10~6 cells were inoculated into 615-type mice by different ways, and the tumor formation rate was 100%. There was no spontaneous remission and its transfer characteristics were preserved. Mycoplasma test negative. Therefore, Ca761/L has a larger application value.