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在自然界往往只有某种形式的旋光异构体才具有生物活性或只有某种光学异构体才有生理作用。如在肽合成、药物合成及不对称化合物的合成工作中都要求对对映体进行有效的分离测定。由于光谱、质谱等方法在对映体的分辨上没有特征,而旋光度法的分辨能力又很低,因此色谱方法,尤其是旋光性固定相的分离方法,显示了优越性。1966年Gil-Av等人首先开发了氨基酸衍生物的旋光性固定相,而后又发展了多种旋光性固定相,如二肽、二胺类及酰脲等等。目前用气相色谱法已成功地分离了氨基酸、胺类、氨基醇类、醣类等对映体,其中Bayer等人提出的以聚硅氧烷为基质的Chirasil-val固定相,因为使用温度较高,选择性较好,目前已成为普遍使用的一种旋光固定相。
In nature, only some form of optical isomers usually have biological activity or only some optical isomers have physiological effects. Such as peptide synthesis, synthesis of drugs and asymmetric synthesis of compounds require an effective separation of enantiomers. Because spectroscopy, mass spectrometry and other methods have no characteristic in the resolution of enantiomers, and the resolving power of the optical rotation method is very low, the chromatographic methods, especially the separation method of the optically active stationary phase, show the superiority. In 1966, Gil-Av et al first developed the optically active stationary phase of amino acid derivatives and then developed a number of optically active stationary phases such as dipeptides, diamines and ureides. At present, the enantiomers of amino acids, amines, amino alcohols, carbohydrates and the like have been successfully separated by gas chromatography. Among them, the silicone-based Chirasil-val stationary phase proposed by Bayer et al. High, the selectivity is better, has become a commonly used optically active phase.