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目的探讨丙烯酰胺(AA)对PC12细胞的细胞毒性及可能机制。方法分别以0、62.5、125、250、500、1 000μmol/L AA为染毒剂量,染毒12 h后,采用MTT比色法检测丙烯酰胺对体外培养PC12细胞的增殖抑制作用,并用碱性单细胞凝胶电泳技术(single cell gel electrophoresis,SCGE)检测PC12细胞DNA的损伤作用、用MDA和T-AOC试剂盒检测细胞MDA和T-AOC改变。结果染毒12 h后,细胞生长受到明显的抑制,且染毒剂量越高,抑制作用越明显(P<0.01);单细胞凝胶电泳实验表明,AA对体外培养PC12细胞的DNA有明显的损伤作用,细胞上清液中MDA含量随着染毒量的上升而升高,T-AOC含量随染毒量的上升而降低。结论 AA能够显著抑制PC12细胞活性,损伤细胞DNA,并诱导细胞氧化损伤。
Objective To investigate the cytotoxicity of acrylamide (AA) on PC12 cells and its possible mechanism. Methods At doses of 0, 62.5, 125, 250, 500 and 1 000 μmol / L, respectively, the effect of acrylamide on the proliferation of PC12 cells was assayed by MTT colorimetric assay after 12 h exposure. Single cell gel electrophoresis (SCGE) was used to detect DNA damage in PC12 cells. The changes of MDA and T-AOC were detected by using MDA and T-AOC kit. Results After 12 h exposure, the cell growth was significantly inhibited and the higher the dose was, the more obvious the inhibitory effect was (P <0.01). The results of single cell gel electrophoresis showed that the DNA of PC12 cells cultured in vitro was significantly The content of MDA in the cell supernatant increased with the increase of the exposure dose, while the content of T-AOC decreased with the increase of the exposure dose. Conclusion AA can significantly inhibit the activity of PC12 cells, damaging the cellular DNA and inducing cell oxidative damage.