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以冰冻保存的绵羊棘球蚴内原头节为抗原作间接荧光抗体试验(IFAT)和间接免疫酶染色试验(IEST),检测对包虫病人、非包虫病人及键康人的敏感性、特异性及交叉反应性,并与间接血凝试验相比较(IHAT)。结果表明IFAT的敏感性和特异性分别为90.36％和97.67％;IEST的敏感性和特异性分别为96.55％和100.0％;两种方法均高于IHAT的敏感性(85.54％)和特异性(95.35％)。三种方法对血吸虫感染者和癌症病人血清均无交叉反应,但对囊虫病人的交叉反应率为IFAT(51.35％)>IHAT(37.84％)>IEST(21.62％)。以原头节为抗原IEST诊断人体包虫病敏感性高、特异性强,适用范围广,不需特殊设备,具有现场应用价值。
The IFAT and IEST were used to detect the antigenicity of hydatid cysts in hydatid cysts of frozen sheep, and their sensitivity to echinococcosis, non-hydatid disease and key human was detected , Specificity and cross-reactivity, compared with the indirect hemagglutination test (IHAT). The results showed that the sensitivity and specificity of IFAT were 90.36% and 97.67%, respectively. The sensitivity and specificity of IEST were 96.55% and 100.0% respectively. Both methods were higher than IHAT sensitivity (85.54%) and specificity 95.35%). There was no cross-reaction between the three methods for sera of schistosomiasis patients and cancer patients, but the rates of cross-reactivity to cysticercosis were IFAT (51.35%)> IHAT (37.84%)> IEST (21.62%). The original head for the antigen IEST diagnosis of human echinococcosis high sensitivity and specificity, wide range of applications, without special equipment, with field value.