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本文在Bruker AM-500 NMR谱仪上,测试了河南桑蚕、辽宁柞蚕(黄茧和人工白茧)丝素在三氟乙酸溶液中的~1H和~(13)C NMR谱,归属了丝蛋白中主要氨基酸的NMR谱线。实验结果显示了桑、柞丝素中氨基酸含量的差异,但柞蚕黄茧和人工白茧丝素的NMR谱图相近。
The ~ 1H and ~ (13) C NMR spectra of silk fibroin from Henan silkworm, Liaoning tussah (yellow cocoon and artificial white cocoon) silk fibroin in trifluoroacetic acid solution were tested on a Bruker AM-500 NMR spectrometer. NMR Spectra of Major Amino Acids in Proteins. The experimental results show the differences of the amino acid content between mulberry and okambe silk fibroin, but the NMR spectra of tussah and artificial white silk fibroin are similar.