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目的观察昆明小鼠摄入不同剂量碘酸钾(KIO3)对血液抗氧化能力的影响。方法将200只昆明小鼠随机分为5个剂量组:低碘组(LI)、适碘组(NI)、5倍高碘组(5HI)、10倍高碘组(10HI)、50倍高碘组(50HI),每组再分为雌雄两个组。LI组饲以低碘地区的粮食制成的饲料(平均碘含量为20~40μg/kg),其它4组饲以正常鼠饲料(平均碘含量为300μg/kg),LI组、NI组饮用去离子水,各高碘组分别饮用含不同浓度(2 023.6、4 553.2、24 789.6μg/L)碘酸钾的去离子水,每只小鼠每日的总摄碘量依次为0.1~0.2、1.5、7.5、15、75μg。喂养3和6个月后,检测抗凝全血的谷胱甘肽过氧化酶(GPx)活力、红细胞超氧化物歧化酶(SOD)活力以及血浆丙二醛(MDA)含量。结果 LI组的GPx活力在3和6个月时较NI组降低,LI组的MDA含量在6个月时较NI组升高,50HI组的MDA含量在6个月时较NI组降低,差异有统计学意义(P<0.05)。雌性组GPx活力、SOD活力和MDA含量与同剂量的雄性组比较,差异无统计学意义。结论碘缺乏会导致大鼠血液抗氧化能力降低,但长期摄入较高剂量的KIO3并未对大鼠血液产生过氧化损伤,而且雌性和雄性间无差异。
Objective To observe the effect of different doses of potassium iodate (KIO3) on antioxidant capacity in Kunming mice. Methods 200 Kunming mice were randomly divided into 5 dose groups: low iodine group (LI), iodine group (NI), 5 times higher iodine group (5HI), 10 times higher iodine group (10HI), 50 times higher Iodine group (50HI), each group is divided into two groups of male and female. The rats in LI group and NI group were fed with diet (the average iodine content was 20 ~ 40μg / kg) and the other four groups were fed with normal rat diet (average iodine content was 300μg / kg) Ionized water and various iodine groups were drenched with deionized water containing different concentrations of potassium iodate (2 023.6, 4 553.2, 24 789.6 μg / L) respectively. The total daily intake of iodine per mouse was 0.1-0.2, 1.5, 7.5, 15, 75 μg. After 3 and 6 months of feeding, the activity of glutathione peroxidase (GPx), erythrocyte superoxide dismutase (SOD) and plasma malondialdehyde (MDA) were detected in anticoagulated whole blood. Results The GPx activity in LI group was lower than that in NI group at 3 and 6 months. The content of MDA in LI group was higher than that in NI group at 6 months. The MDA level in 50HI group was lower than that in NI group at 6 months There was statistical significance (P <0.05). There was no significant difference in GPx activity, SOD activity and MDA content in female group compared with the same dose of male group. Conclusions Iodine deficiency can lead to the decrease of blood antioxidant capacity in rats. However, long-term intake of higher doses of KIO3 did not cause peroxidative damage to blood and there was no difference between female and male.